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Isoflurane inhalation anesthesia should be a new requirement in intracavernosal pressure detection—the gold standard of erectile function assessment

Yuan, J1; Li, J1; Qin, F1

1: West China Hospital, SCU, China

Objectives: Intracavernosal pressure detection is the gold standard for the detection of erectile function in animal experiments, but until now, there is no uniform standard of anesthesia protocols for conducting ICP tests. The ultimate aim of this study was to offer scientific evidence as for which anesthesia protocol is more reliable for ICP test.

Material and Methods: A total of 16 adult male Sprague-Dawley rats were randomized into two groups. In group A, chloral hydrate was injected intraperitoneally. Rats in group B were induced in 5% isoflurane for 3 min and then maintained in 1.0-1.5% isoflurane. Mean arterial pressure (MAP), respiratory rate (RR) and heart rate were monitored during all experiments. After ICP detection, tail vein and carotid artery blood were collected.

Results: The maximum ICP in isoflurane group was significantly higher than in those of chloral hydrate group (109.0 ± 8.9 vs. 54.5 ± 4.5, p = 0.0002). After the MAP was adjusted, the erectile function (presented as ICPmax/MAP × 100) were also shown to be significantly higher in group B (p = 0.0030). During ICP detection, heart rate, respiratory rate and blood pressure were monitored. The values of respiratory rate and blood pressure in group A were less than those in group B, and both parameters were shown to be statistically significant (53.4 ± 3.0 vs. 60.8 ± 1.7, p = 0.0496; 91.6 ± 2.2 vs. 101.6 ± 3.7, p = 0.0383; respectively). The group A had a significantly higher heart rate when compared with group B (365.9 ± 23.8 vs. 300.6 ± 12.2, p = 0.0341). After ICP detection, arterial blood was collected to analyze pO2, pCO2 and saturated oxygen (StO2). Although arterial pO2 in group A was lower and pCO2 was higher compared with group B, this data did not show significant differences between the two groups (p = 0.1602, p = 0.1916, respectively). The value of StO2 for isoflurane group was determined to be statistically different (observed to be higher) from that of the chloral hydrate group (93.0 (91.6; 94.0) vs. 86.9 (84.0; 88.2), p = 0.0084)

Conclusions: Isoflurane inhalation anesthesia in detection of erectile function could offer a relatively more stable physical state than in that under the effect of chloral hydrate intraperitoneal anesthesia. Isoflurane inhalation anesthesia is more suitable for ICP test.


Work supported by industry: no.

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