Effect of liraglutide on streptozotocin-induced diabetic rat testis
Sulejman, S1; Kandirali, E1; Aykan, S1; Çakir, ÖO1; Tüken, M1; Özsoy, S1; Zengi, O1; Gonultas, S1
1: Bagcilar Research And Training Hospital, Turkey
Objective(s): The global prevalence of diabetes mellitus is on rise. Diabetes is associated with changes in testicular tissue and thus affecting reproductive functions. Liraglutide is a glukagon-like peptide-1 receptor agonist and stimulates insülin secretion. The present study is the first to investigate the effect of liraglutide on testis histology and reproductive parameters in streptozotocin-induced diabetic rats.
Materials and Methods: Twenty seven mature male Sprague-Dawley rats were randomly divided into three gropus, i.e, healthy control, diabetic control and liraglutide-treated diabetic rats. Diabetes was induced by two doses of streptozocin in a 3 day period (30 mg/kg; i.p). Treatment group was enjected with liraglutide (Novo Nordisk, Denmark) enjections (0.3mg/kg/12 h s.c) for 12 weeks. Animals were anesthetized by ketamine (60mg/kg/i.p) and xylazine (7.5mg/kg/i.p). Serum levels of total testosterone, LH and FSH were analysed by sandwich enzyme immunoasey method. Testicular tissues were dissected out of a whole via midline abdominal incision and underwent to histological analyses. Testicular damage, apoptosis and neovascularisation were also evaluated by VEGF and Bcl-2 using immunohistochemistry methods.
Results: The biochemichal analysis revealed that the diabetes resulted in significant (p<0.01) reduction in total testosterone and FSH levels but no difference was seen in LH levels. Light microskopic analysis showed remarkable (p<0.01) reduction in seminiferous tubules diameter, Johnsen score, interstitial tissue, testicular weight,VEGF and Bcl-2 expression. Simultaneous administration of liraglutide could fairly up-regulate testosterone and FSH levels. Rats in liraglutide-treated group exhibited with improved tubules atrophy and tubule thickness, elevated Johnsen score and VEGF expression. No difference in Bcl-2 expression was seen between diabetic and liraglutide-treated group.
Conclusion: Our data indicated that Liraglutide therapy could inhibit the diabetes-induced damages in testicular tissue. Moreover administration of liraglutide up-regulated the diabetes-induced FSH and testosterone levels.
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