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abstract

abstract

428

The effect of cavernous nerve traction on erectile function in rats

Li, H1; Chen, Y-W1; Pokhrel, G1; Yang, J1; Wang, T1; Wang, S-G1Liu, J-H1

1: Institute of Urology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, China

Objective. We performed this study to evaluate the effect of cavernous nerve (CN) traction on erectile function in rats.

Material and Method. Thirty-two 8- week-old Sprague–Dawley rats were divided into four groups: control, 1-minute CN traction, 2-minute CN traction, and 2-minute CN crush. CN traction was performed using a glass hook with a tensile force of 0.2 Newton. One month later, the mean arterial pressure (MAP) and intracavernosal pressure (ICP) in response to CN stimulation were measured to assess erectile function. The penis and major pelvic ganglion (MPG) were harvested to explore the expression of neuronal nitric oxide synthase (nNOS) and neurofilament, fibrosis and apoptosis.

Results. The ICP/MAP ratio was reduced in the 2-minute CN traction group compared with the control group (P < 0.05). The ICP/MAP ratio in the CN crush group was lower than in the other three groups (P < 0.05, for each). Expression of nNOS in both MPG and dorsal penile nerve was lower in the CN traction group than in the control group, but was higher than in the CN crush group (P < 0.05). Nerve fiber number in the dorsal penile nerve was reduced by 2-minute CN traction (P < 0.05). The ratios of collagen to smooth muscle content and the apoptosis were both increased the in 2-minute CN traction group compared with the control group (P < 0.05).

Conclusion. CN traction is an effective CN injury model and the injury it caused is relatively mild compared with the CN crush model.

Disclosure:

Work supported by industry: no.

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