Icariin improves SHR erectile function by inhibiting eNOS uncoupling
Jiang, R1; Long, H1
1: Affiliated hospital of Southwest medical University, China
Objective. To investigate whether hypertension has effects on eNOS uncoupling and the effect of icariin on erectile function of SHR is associated with the inhibition of eNOS uncoupling.
Material and Methods. Ten 12-week-old male Wistar-Kyoto (WKY) rats and 10 age-matched male SHR were evenly randomized into four groups (n = 5) , group A: SHR control group (1ml normal saline), group B: SHR+ icariin (10mg/kg•d gavage) treatment group, group C, WKY control group, group D: WKY+ icariin (10mg/kg•d gavage group). Each group was treated with intragastric administration for 4 weeks. Maximum intracavernosal pressure/mean arterial pressure (ICPmax/MAP) was determined. The expression of nitrotyrosine (NT), monomer and dimer of eNOS in the cavernous tissues were determined by Western blotting and the level of BH4, BH2, DHFR, NADPH oxidase and GTPCH1 in corpus cavernosum were determined by enzyme-linked immunosorbent assay (ELISA).
Results. The ICPmax/MAP and the value of BH4, DHFR and GTPCH1 in the icariin treatment group was significantly increased compared with SHR control group (P < 0.05). The value of BH2 and NADPH oxidase was significantly lower in the SHR icariin treatment group than in SHR control group (P < 0.05).The expression of NT and the ratio of eNOS monomer / dimers was significantly lower in SHR icariin treatment group than in SHR control group (P < 0.05).
Conclusion. Hypertension increases eNOS uncoupling in corpus cavernous of SHR. Inhibiting of uncoupling of eNOS may be one of the important mechanisms of icariin to improve SHR erectile function.
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