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Effects of Brazil nut (Bertholletia excelsea) oil-based diet on penile morphology of Wistar rats’

Fernandes-Lima, F1Gallo, C1; Croccia, C2; Miranda, G2; Gregorio, B1; Costa, W1; Sampaio, F1


Objective: We aimed to evaluate if the diet based the oil of Bertholletia excelsa (Brazil nut) is able to reverse morphological changes in the penis of Wistar rats’ cause by an obesogenic diet.

Material and Methods: Wistar rats with 60 days of age were divided into 2 nutritional groups and received the diets until 90 days of age: control group (C, n=30) and high-fat group (lard-based diet), (HF, n=30). At the end of treatment, 10 animals from each group were sacrificed and the remainders were divided into 4 groups of 10 animals each. The groups received their respective experimental diets until 120 days-old: control group (C/C), control - Brazil nut group (C/Nts), high-fat group (HF/HF) and high-fat - Brazil nut group (HF/Nts). At sacrifice (120 days), blood glucose was evaluated and the blood was collected for analysis of total cholesterol (TC), LDL-c, HDL-c and triglycerides. The penises were collected and processed for histomorphometric analyzes of corpora cavernosa area with and without tunica albuginea (CC with TA and CC without TA), and tunica albuginea (TA) area, percentages of connective tissue, smooth muscle and sinusoidal spaces. The results were analyzed by one-way ANOVA and post-Holm-Sidak test, considering p<0.05 as significant.

Results: The HF and HF/HF groups showed increased levels of glucose, CT, HDL-c and LDL-c (p<0.0001). The HF/Nts group showed reduction in glucose, CT and LDL-c levels when compared to HF. Concerning the penis, the cross-sectional areas decrease in HF/Nts (-9.30; p=0.0355) when compared to C-C. CC area with TA: decrease in HF/Nts (-13.68%, p=0.0416) when compared to C-C. CC area without TA decrease in HF/Nts (-9.57%, p=0.0070) when compared to C-C. TA area decreased in HF/Nts (-20.0%; p=0.006) and HF/HF (-14.28%; p=0.0333) when compared to C-C. Connective tissue increase in HF/Nts (+17.61%; p<0.0001) and in C/Nts (+22.13%; p<0.0001) when compared to HF/HF. Smooth muscle decrease in all analyses. Sinusoidal spaces decrease in HF/Nts (-26.55%; p<0.0001) e C/Nts (-27.13%; p<0.0001) when compared to HF/HF.

Conclusion: The results showed that the quality of dietary lipid (Brazil nut oil - source of unsaturated fat) was not enough to reverse morphological changes in the penis induced by the high fat diet. It is likely that a longer exposure time to the Brazil nut oil diet would be necessary to reverse these structural changes.


Work supported by industry: no.

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