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abstract

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Human spermatozoa function is positively influenced by Vitamin D

Silva, P1; Fernandes-Lima, F1Gallo, C1; Sampaio, F1; Sanchez, R2

1: STATE UNIVERSITY OF RIO DE JANEIRO, Brazil; 2: UNIVERSIDAD DE LA FRONTERA

Introduction and Objective: It is reported that vitamin D (VitD) deficiency is associated with male reproductive failure. The objective of this study was to evaluate the direct effect of different concentrations of VitD on the functional parameters of human spermatozoa.

Materials and Methods: Spermatozoa were selected by swim-up from 30 patients, and divided into 5 groups of 6 patients each: Control Group (C) and 4 groups that were exposed to increasing concentrations (nM) of VitD: 0.01VitD; 0.1VitD; 1VitD and 10VitD. Plasma membrane integrity, presence of reactive oxygen species, mitochondrial membrane potential, and loss of plasma membrane cholesterol were assessed by flow cytometry. Calcium transport was observed by confocal microscopy.

Results: Spermatozoa presented better plasma membrane integrity in 1VitD group (64.80 ± 7.94) when compared to groups C (48.52 ± 8.82, p = 0.0150); 0.01VitD (42.40 ± 7.36, p = 0.0015) and 0.1VitD (42.33 ± 8.22, p = 0.0015). 10VitD group (59.65 ± 5.45) also had a greater percentage of spermatozoa with higher plasma membrane integrity in relation to 0.01VitD (42.40 ± 7.36, p = 0.0150) and 0.1VitD (42.33 ± 8.22, p = 0.0150) groups. The production of reactive oxygen species in spermatozoa supplemented with VitD was lower in group 1VitD (15.78 ± 3.04) when compared to groups C (23.48 ± 1.99, p = 0.0042); 0.01VitD (22.53 ± 4.67, p = 0.0115) and 0.1VitD (21.42 ± 4.43, p = 0.0354). The 10VitD group (15.67 ± 0.43) also had lower production of reactive oxygen species in relation to groups C (23.48 ± 1.99, p = 0.0040), 0.01VitD (22.53 ± 4.67, p = 0.0113) and 0.1VitD (21.42 ± 4.43, p = 0.0354). There was no difference in the mitochondrial membrane potential of the spermatozoa among the groups studied. Nevertheless, The loss of plasma membrane cholesterol was higher in all VitD groups [0.01VitD (1.05 ± 0.60); 0.1VitD (2.47 ± 1.77); 1VitD (4.60 ± 2.85) and 10VitD (3.22 ± 292)] when compared to Controls (19.23 ± 4.2, p < 0.0001). The calcium influx was higher in group C (7137.00 ± 4380.00) when compared to group 0.01VitD (1046.00 ± 499.70), as well as in group 10VitD (9309.00 ± 5600.00) when compared to groups 0.01VitD (1046.00 ± 499.70), 0.1VitD (1096.00 ± 389.50) and 1VitD (1160.00 ± 424.40, p = 0.0006).

Conclusion: Vitamin D concentrations of 1.0 nM and 10.0 nM improved the sperm parameters evaluated. These data may suggest that vitamin D in adequate concentrations influences the ability of sperm fertilization, and, therefore, enhances male reproduction. Thus, vitamin D supplementation may represent another possibility for treatment of male fertility disorders.

Disclosure:

Work supported by industry: no.

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