Pericytes in the corpora cavernosa has relationship with endothelial function in hyperlipidemia-associated ED mice
Zhang, Y1; Zhou, M1; Xia, K1; Feng, X1; Gao, Y1; Wan, Z1; Han, D1; Deng, C1
1: 1st affliated hospital of Sun Yat-sen University, China
Objective: To identify if the pericytes in the corpora cavernosa has relationship with endothelial function-tion in experimental hyperlipemia C57BL6 mice.
Methods: The LDLR -/- C57 mice with high fat diet was chosen as the hyperlipidemia ED model. After the model was built, immunofluorescence with the recognized marker of pericytes and the electron microscopy were used to watch the distribution, morphological characteristics of the pericytes in the corpora cavernosa; furtherly, semi-quantitative Western blot was performed to confirm the expression of pericytes in the corpora cavernosa combined with immunofluorescence; Finally, we compared the changes of pericytes and endothelial function to determine whether statistically significant based on the establishment of hyperlipidemia-associated ED models, by which in order to explore the influence of pericytes on endothelial functin and its possible mechanism.
Results: After 12 weeks of feeding fat diet, the total blood cholesterol level, triglyceride level and LDH in hyperlipoidemia group were significantly higher, but the cavernous endothelial function (eNOS by Western blot) was lower than the normal control group, p < 0.005, and it is statistically significant. We found the pericytes through immunofluores cence, electron microscopy and western blot according to the recognized markers, characters of pericytes. Pericytes in the corpora cavernosa were predominately found in the capillary network of the two kinds of microvessels. For example, pericytes are located neaby the endothelial cells of the cavernous sinus for supplying blood for erection and capillary for supplying nutrition to the parenchymal tissue, and P/E is about 1 : 3. Finally, we found that the changes of pericytes (Morphology, number, immunofluorescence intensity and western blot) related to endothelial dysfunction in hyperlipidemia-associated ED mice.
Conclusion: We found that changes of pericytes related to the endothelial functiontion in experimental hyperlipemia C57BL6 mice, which suggest that the pericyte may play an important role in the erection.
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