Stem cell conditioned media profiles and potential uses in erectile dysfunction
Matz, EM1; Dou, LD2; Zhang, YZ2; Terlecki, RT3; Atala, AA2; Jackson, JJ2
1: Wake Forest Baptist Medical Center, United States; 2: Wake Forest Institute for Regenerative Medicine; 3: Wake Forest Baptist Medical Center
Objective: Proposed mechanisms of benefit of stem cells for erectile dysfunction vary. The prevailing theory involves release of trophic factors, producing a favorable microenvironment for regeneration. We measured trophic factors expressed by placental derived stem cells (PSCs), amniotic fluid derived stem cells (AFSCs), adipose derived stem cells (ADSCs) and endothelial cells (ECs) to characterize factor profiles possibly relevant to changes seen with in vivo administration.
Materials and Methods: All cell lines were cultured (ADSC, AFSC, PSC, EC) in duplicate. ADSC were used at passage less than 5, PSC were used at passages between 7 and 12, AFSC were used at passages between 5 and 12 and endothelial cells were used at passages less than 5. All cells were grown to 60-70% confluence and incubated in serum-free media for 24 hours. Conditioned media was removed and concentrated 10-20 fold. All samples were sent for 200 human biomarker analyses (Ray Biotech, Norcross, GA). Heat maps and principal component analysis (PCA; Qlucore Omics Explorer http://qlucore.com/) were then generated.
Results: Based upon heat maps and PCA, ADSC and AFSC were most similar in expression (both substantially different from PSC). The patterns for EC were unique from stem cell lines. Using Ingenuity pathway analysis (Qiagen, Germantown, MD), pathways involving cell proliferation and cell-to-cell signaling seem differentially expressed.
Conclusion: Among mesenchymal stem cells, PSC factor expression was unique. These results may offer insight into differential results of in vivo administration. Strong secretome profiles within conditioned media may provide an injectable cell or cell-free therapeutic.
Work supported by industry: no. The presenter or any of the authors act as a consultant, employee (part time or full time) or shareholder of an industry.Go Back