Fetal programming by high-fat diet promoted prostatic atrophy in Wistar rats’ offspring
Campos-Silva, P1; Souza-Fernandes, A1; Souza, D1; Gallo, C1; Costa, W1; Sampaio, F1; Gregorio, B1
1: State University of Rio de Janeiro, Brazil
Objective: Evaluate the effects of high-fat diet intake during pregnancy, lactation and/or postnatal period on the prostate morphology of Wistar rats at 4 months-old.
Material and Methods: Twenty female Wistar rats were fed with control diet (C) or high-fat diet (HF), during pregnancy and lactation. After birth, male offspring were divided into 4 groups as following: C/C – offspring of dams fed C during gestation and lactation, maintaining a C diet at postnatal period, C/HF – offspring of dams fed C during gestation and lactation, changing to HF diet at postnatal period, HF/C - offspring of dams fed HF during gestation and lactation, changing to C diet at postnatal period and HF/HF - offspring of dams fed HF during gestation and lactation, maintaining a HF diet at postnatal period. Biometric (food intake, body weight) and metabolic parameters (oral glucose tolerance test, serum glucose, total cholesterol, triacylglycerol, insulin and testosterone levels) were assessed. At euthanasia, the ventral prostate was dissected and prepared for histomorphometrical analysis. Data were analyzed by unpaired t-test, one-way ANOVA and Bonferroni posttest.
Results: Dams did not present any difference on body mass gain and energy intake during the perinatal period. Also, no differences were found in insulin and total cholesterol levels in the offspring among groups. Postnatal HF diet lowered testosterone serum levels in comparison to C/C group (p=0.0218). Administration of HF diet on perinatal period (HF/C group) promoted an elevation on triacylglycerol levels in comparison to C/C (p=0.0005). Also, serum glucose was higher in this group and in HF/HF when compared to controls (p=0.0017). Regarding pups prostate, the acinar area was lower in all groups, compared to C/C (p<0.0001); epithelium height was decreased in the HF/C and HF/HF, compared to C/C and C/HF groups (p<0.0001); smooth muscle cells area density was reduced by the peri or postnatal HF diet; connective tissue area density was negatively influenced by perinatal and postnatal HF diet, when compared to controls (p=0.0013; p<0.0001, respectively) and epithelium area density was lower in HF/C than C/C and C/HF groups (p=0.0024).
Conclusion: The HF diet during gestation, lactation, and/or the postnatal period promoted hyperglycemia and prostatic atrophy. These data suggest that a HF diet could be promoted an impairment of the secretory activity and contractility the prostate, which may impair its activity in adulthood.
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