Serum reactivity to human Hsp60 in women with pelvic inflammatory diseases and sexual dysfunctions
Yakovenko, L1; Romashchenko, O2; Biloholovska, V2; Kroupskaya, I1
1: Institute of Molecular Biology and Genetics, National Academy of Sciences of Ukraine, Ukraine; 2: SI 'Institute of Urology of the NAMS of Ukraine', Ukraine
Objective. To investigate the humoral reactivity to human Heat shock protein 60 (hHsp60) in women with pelvic inflammatory diseases (PID) and sexual dysfunctions as a risk marker of disorders of reproductive health.
Material and Methods. The study included 61 women (26-48 years) with PID of duration 2-15 years. Ultrasonography of anatomic structures of small pelvic organs and the evaluation of functional indicators of clitoris zones have been carried out by Doppler method. Serum reactivity to hHsp60 has been identified in 16 women with PID and dyspareunia by ELISA and Western blotting. The serum samples of women without reproductive and sexual disorders were used as control.
Results. Dyspareunia with varying degrees of disease severity have been established in 55.73% (34/61) women. It has been combined with disorders of reproductive function in 82.35% (28/34) women: infertility has been observed in 55.88% (19/34), miscarriages – in 23.53% (8/34), and ectopic pregnancy – in 2.94% (1/34) cases. The increase of indices of resistance (IR=0.78-0.85) with simultaneous the reduction of a speed of voluminous blood flow (Vs=6.5±9.0 cm/sec) have been shown in women with dyspareunia and disorders of reproductive function by Doppler method. Antibodies to hHsp60 have been detected in all serum samples investigated. There were significant differences in anti-hHsp60 antibody levels between women with dyspareunia and control (p<0.001). High serum reactivity to hHsp60 has been detected in 12.5% (2/16) and 75.0% (12/16) women with dyspareunia by ELISA and by Western blotting respectively. The target antigen hHsp60 has been recognized by serum from women with dyspareunia and miscarriages or ectopic pregnancy by Western blotting. Affinity purified IgG anti-Hsp60 antibodies from highly reactive to Hsp60 serum samples of women with disorders of reproductive function recognized both prokaryotic Hsp60 and hHsp60 by Western blotting. High serum reactivity to hHsp60 may be mediated not only by anti-hHsp60 antibodies itself but also by the cross-reactivity of anti-bacterial Hsp60 antibodies presented in serum of women with PID.
Conclusion. Formation of dyspareunia in women with PID is combined with the disturbance of reproductive function and requires adequate timely correction. Serum reactivity to hHsp60 may be an additional diagnostic risk marker of disorders of reproductive function in women with dyspareunia.
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