Potential role of 5-HT serotonin system in the rat spinal cord of premature ejaculation with category III prostatitis
Zhang, Y1; Zheng, T1; Xia, K1; Yang, Q1; Zhou, M1; Tu, X1; Sun, X1; Deng, C1
1: 1st affliated hospital of Sun Yat-sen University, China
Objectives: To explore the mechanism of premature ejaculation in rat with category III prostatitis, or chronic prostatitis/chronic pelvic pain syndrome (CP/CPPS).
Methods: We establish rat model of experimental autoimmune prostatitis (EAP) by subcutaneous multiple point's injection of male prostate gland extract emulsified in an equal volume of Freund’s adjuvant at the 0 and 21th day. The behavioral change and the sexual behavior was investigated. Then, We took out the hippocampus tissue, T13-L2 and L5- S2 spinal cord tissue after perfusion, and detected the levels of 5-HT, 5-HT1A receptor, 5-HT2C receptor and SERT in these tissues by immunohistochemical staining. The levels of 5-HT, 5-HT1A receptor, 5-HT2C receptor and SERT in the remaining rats were detected by Western Blot.
Results: The EAP model was built successfully. Serum IL- 1β and TNF-α± levels in the model were significantly higher than control (p<0.01). In the copulatory test, compared with normal control group, mount frequency (MF), intromission frequency (IF) and ejaculation frequency (EF) in the model group were significantly decreased (p <0.05); mount latency (ML), intromission latency (IL) and postejaculatory interval (PEI) in the model group were significantly shortened(p <0.05); ejaculation latency (EL) in the model group was significantly significantly prolonged (p <0.05). In hippocampus tissue, 5-HT levels in the model were significantly lower than normal (p<0.05), whereas 5-HT1A, 5-HT2C and SERT levels in the model were significantly higher than normal (p<0.05). In T13-L2 spinal cord tissue, 5-HT levels in the model were significantly lower (p<0.05), 5-HT1A receptor levels in the model were significantly increased (p<0.05). In L5-S2 spinal cord tissues, 5-HT levels in the model were significantly lower (p<0.05), while the 5-HT1A, 5-HT2C and SERT levels in the model increased slightly, but the differences in 5-HT1A, 5-HT2C and SERT levels between the two group were statistically significant (p<0.05).
Conclusions: A rat model of EAP that exhibited characteristics of depression, decreased libido and premature ejaculation was successfully established. 5-HT neurotransmitter system may plays an important role in rat through spinal cord as in the brain.
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